Figure 7.

Human purified C1INH dose dependently reduces WB TG and normalizes venous thrombus formation in C1INH-deficient mice. Purified human C1INH was added to WB from C1inh^−/− mice at increasing concentrations ex vivo and the effect on silica-initiated WB TG compared with vehicle control. (A) Representative thrombin silica-initiated WB TG curves were plotted and (B) TG lag time and (C) peak TG quantified. Data are represented as individual values with mean ± standard error of the mean and analyzed by one-way ANOVA with post hoc Bonferroni. (D) C1inh^−/− mice were administered purified human C1INH or vehicle control and VT induced using the IVC stenosis model. Schematic of C1INH VT rescue experiment. (E) Thrombus weight and (F) thrombus incidence was assessed 24 hours after induction. Thrombus weight data are represented as individual values with median and analyzed using Kruskal-Wallis with post hoc Dunn’s, and thrombus incidence data are analyzed using Fisher exact test. ∗P < .05, ∗∗P < .01, ∗∗∗P < .001, ∗∗∗∗P < .0001. ANOVA, analysis of variance.