FIGURE 2.

(a) Activity scores for 3929 of Src catalytic domain variants in two radicicol‐treated replicates. (b) Linear regression of activity scores and individually measured growth rates (n = 3) of 13 variants in either radicicol‐ or DMSO‐treated conditions. These linear models were used to convert radicicol activity scores to calibrated radicicol activity scores, which were then used to calculate a client score for each variant by subtracting the DMSO activity score from the calibrated radicicol activity score. (c) Individually measured growth rates of yeast expressing nonclient Src_K298M, weak client Src_WT, or functionally dependent client Src_F512P in either basal or Hsp90‐inhibited conditions (n = 3), along with each variant's client score. (d) Distribution of nonsynonymous (n = 3366, gray), synonymous (n = 430, green), and nonsense (N = 133, red) client scores of variants in the library. Dashed line indicates the client score used to define functionally dependent clients. Functionally dependent client variants E381R and T341I are indicated by arrows. The client score of kinase‐dead variant K298M and the apparent client score of empty vector are indicated by arrows over the nonsense distribution.