| Gene name | Forward primer | Reverse primer |
|---|---|---|
| Ets | ACTATGAGAAGCTGAGCCGC | TTGCCGAGTTCTCAGTCGTC |
| Eve | TGGACTCGACAACCACTTCG | GACGACAAGCCCTATACCCG |
| FoxA | TTCATCCTGACGCTGGCAAT | CGTTAGGGTCTCCGTTCTCG |
| FoxB | TGAGCGCCCGACAAACTTAT | TTTACTGCCTTGGCCTCTGG |
| GATAc | CATGTTCCTGCGGCTTATGC | ATTCTCGGCCCTCTGTTGTG |
| Gcm | TCACAGCCTCACAAGACGAC | TGCCATCGTACCCGTAATCG |
| Gsc | CGGATTCTGACGGGCTAGAC | GTGAACGGGATAGCGAGAGG |
| Hnf6 | CGAAAGAAATCGCAGCGAGG | ACTTCCACATCCGCCTGAAG |
| Blimp1b | GATTGCATCCCGACCTCGAT | CGGAAAATCCTTGAGGGGCT |
| Msp130 | TGTTGGAGGTCGTCGACTTG | TTTAGCGAGCCAACATCCGT |
| Nkx2.1 | GCCACGCAATCCATGACATC | GCTTGGCTTTGAGGGTGTTG |
| Nodal | GACATGGCAGACCTCCTTCC | ACGGGTGGTCGAAGGTAGTA |
| Not | TAAATTCCACCTGCCCGCTT | GGCGATGGATGGAAGACGAT |
| Pks1 | TCTGCATCACACACCAGGAC | ACGCACAAGAGGTCTCCAAG |
| Pmar1 | CCGTCGAGCCTCTCTTGTTT | CACGACGCCCAACTTCTTTG |
| SoxB1 | TCTATCCCAGGCATGACCCA | TCCTGCGTTGTCCTCTTGAC |
| SoxE | GACAAGGAGAAGCAGCCCTT | CAAGGTGGAGCGGTTGTTTG |
| Tbr | AGGCGTCAGTTTACCTCTGC | CCACTGGTTCGGATCACACA |
| Wnt8 | TCAGCGGGAAACTCATCGAC | CTGATGCCGATGGTCAGGTT |
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