Fig. 3.

Overexpressed miR-5195-3p inhibited proliferation, migration, invasion, and EMT in HepG2/IR cells. (A). Relative miR-5195-3p expression levels in HepG2/IR cells were detected using qRT‒PCR after transfection with miR-5195-3p mimic or its control (NC mimic). MTT assays (B), colony formation assays (C), and EdU assays (D) were employed to detect the proliferation of HepG2/IR cells after transfection with the NC mimic or the miR-5195-3p mimic. Representative images of migration and invasion (E) and scratch wound healing assays (F) of HepG2/IR cells transfected with miR-5195-3p mimic or NC were determined. G. The protein expression of E-cadherin, N-cadherin, Vimentin and Snail in HepG2/IR cells transfected with miR-5195-3p mimic was determined by Western blots; the membranes were cut prior to hybridization with antibodies. The experiments were independently repeated three times. (* P < 0.05, ** P < 0.01)