Fig. 7.

miR-5195-3p directly targeted the 3′-UTR of SOX9 and TPM4. The predicted targeting sites of SOX9 and TPM4 (A and B). A dual luciferase reporter assay was performed to verify the direct target of miR-5195-3p (C and D). (E). Western blotting was performed to detect the protein expression levels of SOX9 and TPM4 in HepG2 cells transfected with NC inhibitor or miR-5195-3p inhibitor, and HepG2/IR cells were transfected with the NC mimic or miR-5195-3p mimic; the membranes were cut prior to hybridization with antibodies. The experiments were independently repeated three times. (* P < 0.05, ** P < 0.01)