GPC3 is expressed on human NE cells of the prostate. (A) mRNA-seq results of FACS-sorted luminal and NE cells from prostatic adenocarcinoma (n = 3) demonstrating GPC3, CHGA, and KLK3 expression. (B) sc-RNA seq results from human PCa cases (n = 6) showing GPC3, SYP, and NCAM1 expression in different cell clusters throughout the spectrum of PCa. (C) Representative IHC image showing GPC3 expression in CRPC-adeno and SCNC (scale bar, 50 μm). (D) IF images showing GPC3 and CgA co-expression in CRPC-adeno tissue. (E) Circle chart demonstrating percentage of SCNC cases that were positive for GPC3 by IHC. Statistics: t-test with p < 0.05 considered significant. p < 0.05 (*).