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GPC3 regulates [Ca2+]i to control CAMKII activation in SCNC. (A) Quantification of MFI values from Cal-520 AM assays demonstrating [Ca2+]i in NCI-H660/shCTRL versus NCI-H660/shGPC3, LNCaP/CXCR2/shCTRL versus LNCaP/CXCR2/shGPC3 and LNCaP/EV versus LNCaP/GPC3. (B) Western blot analysis showing CAMKII activation status in NCI-H660/shCTRL versus NCI-H660/shGPC3. Statistics: t-test with p < 0.05 considered significant. p < 0.05 (*), p < 0.01 (**), p < 0.0001 (***).
