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Efficacy of the SCFluo method when performed on sheared plasmid samples. (A) Fluorescence of ambient (open symbols) and heated (95°C)–cooled (closed symbols) aliquots of pQR186 (circles) and pQR150 (triangles) samples as a function of shear rate. (B and C) Comparison of SC% obtained from densitometric scanning of an agarose gel (closed bars) and the SC% derived from equation 9 (open bars) for pQR186 and pQR150, respectively.
