Skip to main content
. 2000 Jun 15;28(12):e59. doi: 10.1093/nar/28.12.e59

Figure 1.

Figure 1

Description of the method presented in this paper for monitoring in vitro transcriptional RNA synthesis. Two fluorescent oligoDNAs are prepared, each labeled with a fluorescent molecule different from the other (donor probe and acceptor probe). When the two oligoDNA probes hybridize to an adjacent sequence on the target RNA, FRET occurs (inset). Excess amounts of two fluorescent oligoDNA probes complementary to a site in the synthesized RNA are added to the in vitro transcription reaction solution and the reaction carried out. Fluorescent probes hybridize to the synthesizing RNA molecules while the reaction is progressing. When a hybrid with a donor probe, an acceptor probe and an RNA molecule is formed, FRET occurs and the hybrid emits a distinctive fluorescence signal in the presence of other non-hybridizing probes.