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. 2000 Jun 15;28(12):e59. doi: 10.1093/nar/28.12.e59

Figure 3.

Figure 3

OligoDNAs for different sites hybridize to xelf1-α RNA with different efficiency. (A) Fluorescence spectra of the mixture of xelf1-α RNA and a probe pair for each site at a molar ratio of 1:1. For each specific 30mer site of xelf1-α RNA, a probe pair (Bodipy493/503-labeled 15mer DNA and Cy5-labeled 15mer DNA) was prepared. The fluorescence spectra of the five kinds of probe pairs were the same and only one spectrum (probes for the 184–213 site) is shown. (B) Ratios of fluorescence intensity at 670 and 514 nm (I670 nm/I514 nm) of the spectra in (A). The I670 nm/I514 nm value of a mixture of the probe pair for 184–214 and the 30mer complementary RNA (Fig. 2) is also shown (30mer RNA).