Figure 5.

Monitoring of in vitro transcription reactions by fluorescence spectral changes using probes for various sites. Two other probe pairs (for the 184–214 and 1504–1534 sites) were used in monitoring experiments as described in Figure 4A–C. Ratios of fluorescence intensity (I_{670 nm}/I_{514 nm}) are plotted against incubation times. (A) The vector containing xelf1-α cDNA was used as template. (B) The vector containing a partial length of xelf1-α cDNA, which was deleted 0.96 kb from the 3′-end, was used as template. Using this template, the synthesized RNA had an ~1.1 kb length (confirmed on agarose gel electrophoresis) and had no site for probes that were complementary to the 1504–1534 site.