Table 1. Effect of numbers of probes on monitoring.
| Probe/template molar ratio | Fluorescence spectra | Absorbance | ||
|---|---|---|---|---|
| I670/I514 | Target RNA/probe (molar ratio of hybridizing probes) | Number of hybrids (per template) | Number of transcripts (per template) | |
| 200 | 0.40 | 0.68 | 136 | 309 |
| 800 | 0.17 | 0.31 | 248 | 298 |
| 1200 | 0.12 | 0.22 | 264 | 273 |
The results of Figure 6 are summarized using the values at 180 min. For estimating the number of transcripts from absorbance at 260 nm, 1 absorbance unit was estimated to be equivalent to 40 µg of RNA and 622 800 was used as the molecular weight of xelf1-α RNA.