Figure 3.

DIC and fluorescence micrographs of MRSA-USA100 NRS705 cells treated for 3 h with DMSO vehicle (A and B), 4 μg/mL TXA707 (C and D), or 1 μg/mL TXH9179 (E and F). The cells were also labeled with the fluorescent FtsZ probe BOFP to yield the fluorescence micrographs shown in B, D, and F. The white arrows in B highlight normally dividing cells with FtsZ localized to the midcell septa. The red arrows in B highlight cells presenting in a transverse orientation that reveal the circular nature of the FtsZ Z-ring. The yellow arrows in D and F highlight enlarged cells resulting from TXA707 or TXH9179 treatment in which FtsZ is mislocalized to one or more large foci at the cell periphery. Scale bars reflect 1 μm.