Table 3.
Top 30 of 543 non-MyoF proteins different between Y versus MA participants
| Protein (gene symbol) | Y (n=5) | MA, pre- (n=6) | p-value |
|---|---|---|---|
| Top 15 of 96 non-MyoF proteins higher in Y versus MA (p<0.01) | |||
| Isoform 2 of Glycerol-3-phosphate dehydrogenase, cytoplasmic (GPD1) | 1984 ± 263 | 1067 ± 510 | 0.0026 |
| Protein NDRG2 (NDRG2) | 1428 ± 200 | 903 ± 268 | 0.0031 |
| Acylphosphatase-2 (ACYP2) | 564 ± 142 | 329 ± 92 | 0.0076 |
| Aspartate aminotransferase, cytoplasmic (GOT1) | 478 ± 54 | 94 ± 48 | 0.0068 |
| Isoform 3 of Exportin-2 (CSE1L) | 443 ± 79 | 256 ± 102 | 0.0031 |
| Beta-enolase (ENO3) | 378 ± 63 | 240 ± 49 | 0.0024 |
| Acyl-coenzyme A thioesterase 2, mitochondrial (ACOT2) | 351 ± 61 | 195 ± 84 | <0.0001 |
| Aspartate aminotransferase, mitochondrial (GOT2) | 267 ± 87 | 57 ± 33 | 0.0001 |
| GTP:AMP phosphotransferase AK3, mitochondrial (AK3) | 264 ± 54 | 132 ± 54 | 0.0004 |
| Adenylosuccinate lyase (ADSL) | 239 ± 22 | 135 ± 52 | 0.0033 |
| Acyl-coenzyme A thioesterase 1 (ACOT1) | 202 ± 61 | 85 ± 42 | 0.0006 |
| Guanidinoacetate N-methyltransferase (GAMT) | 202 ± 50 | 97 ± 22 | 0.0004 |
| Isoform 3 of UV excision repair protein RAD23 homolog A (RAD23A) | 161 ± 37 | 93 ± 28 | 0.0061 |
| Malate dehydrogenase, cytoplasmic (MDH1) | 140 ± 53 | 47 ± 27 | 0.0046 |
| Carboxymethylenebutenolidase homolog (CMBL) | 131 ± 23 | 70 ± 25 | 0.0041 |
| Top 15 of 447 non-MyoF proteins higher in MA versus Y (p<0.01) | |||
| Phospholamban (PLN) | 1212 ± 262 | 2378 ± 572 | 0.0024 |
| Calsequestrin-1 (CASQ1) | 1281 ± 218 | 2344 ± 670 | 0.0082 |
| Trifunctional enzyme subunit beta, mitochondrial (HADHB) | 861 ± 98 | 1561 ± 397 | 0.0041 |
| Trifunctional enzyme subunit alpha, mitochondrial (HADHA) | 756 ± 100 | 1292 ± 335 | 0.0075 |
| Isoform 2 of Sarcalumenin (SRL) | 296 ± 69 | 593 ± 181 | 0.0074 |
| Troponin C (TNNC1) | 291 ± 46 | 494 ± 123 | 0.0071 |
| Troponin I (TNNI1) | 219 ± 28 | 466 ± 145 | 0.0047 |
| Myomesin-2 (MYOM2) | 95 ± 36 | 349 ± 127 | 0.0020 |
| PRA1 family protein 3 (ARL6IP5) | 214 ± 36 | 322 ± 58 | 0.0059 |
| Tropomyosin alpha-3 chain (TPM3) | 129 ± 21 | 295 ± 76 | 0.0011 |
| Complement C1q subcomponent subunit C (C1QC) | 91 ± 8 | 251 ± 49 | <0.0001 |
| Calpain-1 catalytic subunit (CAPN1) | 155 ± 11 | 213 ± 26 | 0.0013 |
| Complement C1q subcomponent subunit B (C1QB) | 74 ± 9 | 206 ± 48 | 0.0002 |
| Mitochondrial import receptor subunit TOM40 homolog (TOMM40) | 83 ± 13 | 206 ± 63 | 0.0023 |
| Isoform 3 of Hexokinase-1 (HK1) | 134 ± 28 | 204 ± 29 | 0.0030 |
Data are presented as mean ± standard deviations for individual protein spectra values (normalized to total run spectra values) in younger (Y) and pre-intervention middle-aged (MA) participants. Protein targets more abundant in Y versus MA are sorted from most to least abundant in the Y cohort. Protein targets more abundant in MA versus Y are sorted from most to least abundant in the MA cohort.