Fig. 4. CAP-TAC1 is a potent tachykinin receptor agonist.

(A) Schematic and annotation of the primary amino acid sequence for full-length mouse TAC1 preproprotein and its cleavage products. For (B-D), chemiluminescent signal intensity in PathHunter beta-arrestin CHO-K1 cells transfected with human (B) TACR1, (C) TACR2, and (D) TACR3 following treatment with the indicated concentration of CAP-TAC1 or substance P (SP). (E) Relative levels of CAP-TAC1 or substance P (SP) in mouse plasma following incubation at 37°C for the indicated time. For (B-D), N=2 per concentration; for (E), N=3 per condition. For (B-D), data are shown as means ± SEM. * P < 0.05, ** P < 0.01 versus control by Student’s t-test.