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[Preprint]. 2023 Jun 7:2023.06.02.543503. [Version 2] doi: 10.1101/2023.06.02.543503

Fig. 5. Functional studies of CAP-GDF15 in feeding and energy balance in mice.

Fig. 5.

(A) Schematic of the primary amino acid sequence for full-length mouse GDF15 preproprotein and annotation of CAP-GDF15 sequence as well as the canonical GDF15 hormone sequence. (B, C) Representative extracted ion chromatogram (B) and mirror tandem fragmentation spectra (C) of CAP-GDF15 (pGlu-LELRLRVAAGR-NH2; +2 ion m/z = 682.4) and the endogenous plasma peak. (D-F) Food intake (D), respiratory exchange ratio (RER, E), and ambulatory activity (F) of 12–16-week diet-induced obese male mice following a single treatment of CAP-GDF15 (50 mg/kg, intraperitoneal) or vehicle control. (G-I) Food intake (G), RER (H), and ambulatory activity (I) of 12–16-week diet-induced obese male mice following a single treatment of scrambled CAP-GDF15 (50 mg/kg, intraperitoneal, scrambled sequence: pGlu-GLEALRARLRV-NH2) or vehicle control. (J, K) 3-day food intake (J) and body weight (K) of 12–16-week diet-induced obese male mice following treatment with CAP-GDF15 or scrambled CAP-GDF15 (50 mg/kg/day, IP), or vehicle control. Data are shown as means ± SEM. For (D-F), N=12/group; for (G-I), N=8/group; for (J,K), N=7/group. For (D-I), injection occurred at time T=0 (5:00pm) and data was collected for the following 16 hrs. For (D-K), ** P < 0.01, *** P < 0.001 by two-way ANOVA.