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. 2000 Jun 15;28(12):e62. doi: 10.1093/nar/28.12.e62

Figure 2.

Figure 2

SYBR Green I stained polyacrylamide gels (12% PAGE in A and B, 8% PAGE in C) showing the efficacy of the purification step in the microSAGE procedure for untreated PC12 cells. Prior to the use of Clontech spe10 spin gel filtration columns, 26 bp ditags are not detectable (A). As shown above in Figure 1 for SAGE, DNA purification through the columns remarkably improves NlaIII digestion (B) and facilitates formation of concatemers (C). M1, 20 bp DNA ladder; M2, 100 bp DNA ladder; M3, 1 kb DNA ladder.