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[Preprint]. 2023 Jun 9:2023.06.07.544098. [Version 1] doi: 10.1101/2023.06.07.544098
Component 1 reaction (ul)
Nuclease-free water Variable
Template RNA* Variable
100uM SMART_dT18VN 0.5
10mM dNTPs 1.0
Total 14.0
*
Application note: Up to 5ug total RNA can be used for cDNA synthesis. It is recommended to maximize the starting RNA amounts to maximize recovery of Calling Cards insertions.