Figure 5. Interaction at the level of expression between AtrR and FfmA.

A. Isogenic wild-type and atrRΔ strains were grown and whole cell protein extracts prepared. Equal amounts of these extracts were analyzed by SDS-PAGE using either anti-AtrR or anti-FfmA antisera. B. The DO-ffmA-containing strain was grown either in the presence (+) or absence (−) of doxycycline for 12 hours. Whole cell protein extracts were analyzed as described above using the same antibodies. Molecular mass standards are indicated in kD on the left.