Table 1:

Sorbitol concentration (mol/L) at which protoplasts reach isotonic state compared to intact cells for the three different experimental approaches.

Methods╲Strains	S. pombe (mol/L)	S. japonicus (mol/L)	S. cerevisiae W303 (mol/L)	S. cerevisiae BY4741 (mol/L)
Cellular volumes	0.39± 0.4	0.19± 0.02	0.25± 0.03	0.13± 0.02
Cytoplasmic concentration	0.37± 0.3	0.21 0.03	0.16± 0.01	0.10± 0.01
Rheology	0.43± 0.02	n/a	0.20± 0.01	0.13± 0.01
Average values of these three methods	0.40± 0.02	0.20± 0.01	0.20± 0.04	0.12± 0.01

Summary of the sorbitol concentration added to the media for protoplasts to match the internal osmotic potential of intact cells for the three methods (see Methods). Last row gives the average values from the three methods (Mean ± SD).