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[Preprint]. 2023 Jun 7:2023.06.07.544135. [Version 1] doi: 10.1101/2023.06.07.544135

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Figure 3. — (A) Schematic of AUG-V5-(G4C2)₆₉-NLuc-3xFLAG in GA frame and AUG-V5-+1(CGG)₁₀₁-NLuc-3xFLAG in polyG frame. (B) FLAG and V5 IP workflow to enrich for incomplete products generated from GC-rich transcripts. 3xFL: 3xFLAG (C-D) V5 antibody IP reveals short/stalled products (line next to the blot) that are generated from G4C2 (panel C) or CGG repeats (panel D) that do not contain the carboxyl-terminal FLAG tag. FT: Flow-through.