[Preprint]. 2023 Jun 7:2023.06.07.544135. [Version 1] doi: 10.1101/2023.06.07.544135

Table 3.

DNA plasmid sequence used to synthesize RNA transcript

Reporter name	Sequence from T7 to PspOMI cut site
ATG-V5-NLuc-3xFLAG	GGAGACCCAAGCTGGCTAGCGTTTAAACTTAAGCTTGGCAATCCGGT ACTGTTGGTAAAGCCACCATGGGTAAGCCTATCCCTAACCCTCTCCTC GGTCTCGATTCTACGGTCTTCACACTCGAAGATTTCGTTGGGGACTGG CGACAGACAGCCGGCTACAACCTGGACCAAGTCCTTGAACAGGGAG GTGTGTCCAGTTTGTTTCAGAATCTCGGGGTGTCCGTAACTCCGATCC AAAGGATTGTCCTGAGCGGTGAAAATGGGCTGAAGATCGACATCCAT GTCATCATCCCGTATGAAGGTCTGAGCGGCGACCAAATGGGCCAGAT CGAAAAAATTTTTAAGGTGGTGTACCCTGTGGATGATCATCACTTTAA GGTGATCCTGCACTATGGCACACTGGTAATCGACGGGGTTACGCCGA ACATGATCGACTATTTCGGACGGCCGTATGAAGGCATCGCCGTGTTC GACGGCAAAAAGATCACTGTAACAGGGACCCTGTGGAACGGCAACAA AATTATCGACGAGCGCCTGATCAACCCCGACGGCTCCCTGCTGTTCC GAGTAACCATCAACGGAGTGACCGGCTGGCGGCTGTGCGAACGCATT CTGGCGGACTACAAAGACCATGACGGTGATTATAAAGATCATGACATC GATTACAAGGATGACGATGACAAGTAAGGCCGCGACTCTAGAG
ATG-V5-GA₆₉-NLuc-3xFLAG	GGGAGACCCAAGCTGGCTAGCTAGCTAGTAGATGGGTAAGCCTATCC CTAACCCTCTCCTCGGTCTCGATTCTACGGGATATCAAGATAGCGCCG GGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGG GGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCAGGGGCCGGG GCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGG CCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCG GGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGG GGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGG GCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGG CCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC GGGGCCGGGGCCGGGGCCGGGGCCGGGCTGGAAGCTTGGCAATCC GGTACTGTTGGTAAAGCCACCGGGGTCTTCACACTCGAAGATTTCGTT GGGGACTGGCGACAGACAGCCGGCTACAACCTGGACCAAGTCCTTG AACAGGGAGGTGTGTCCAGTTTGTTTCAGAATCTCGGGGTGTCCGTA ACTCCGATCCAAAGGATTGTCCTGAGCGGTGAAAATGGGCTGAAGAT CGACATCCATGTCATCATCCCGTATGAAGGTCTGAGCGGCGACCAAA TGGGCCAGATCGAAAAAATTTTTAAGGTGGTGTACCCTGTGGATGATC ATCACTTTAAGGTGATCCTGCACTATGGCACACTGGTAATCGACGGG GTTACGCCGAACATGATCGACTATTTCGGACGGCCGTATGAAGGCAT CGCCGTGTTCGACGGCAAAAAGATCACTGTAACAGGGACCCTGTGGA ACGGCAACAAAATTATCGACGAGCGCCTGATCAACCCCGACGGCTCC CTGCTGTTCCGAGTAACCATCAACGGAGTGACCGGCTGGCGGCTGTG CGAACGCATTCTGGCGGACTACAAAGACCATGACGGTGATTATAAAG ATCATGACATCGATTACAAGGATGACGATGACAAGTAAGGCCGCGAC TCGAGAG
ATG-V5-+1(CGG)₁₀₁-NLuc-3xFLAG	GGAGACCCAAGCTGGCTAGCGTTTAAACTTAAGCTTGGTACCGAGCT CGGATCCACTAGTCCAGTGTGGTGGAATTCGTAGTATGGGTAAGCCT ATCCCTAACCCTCTCCTCGGTCTCGATTCTACGGCGCCGCTGCCAGG GGGCGTGCGGCAGCGCGGCGGCGGCGGCGGCGGCGGCGGAGGCG GCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGG CGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGC GGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCG GCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGG CGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGC GGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCG GCGGCGGCGGCGGCGGCTGGGCCTCGAGGATATCAAGATCTGGCCT CGGCGGCCAAGCTTGGCAATCCGGTACTGTTGGTAAAGCCACCGGG GTCTTCACACTCGAAGATTTCGTTGGGGACTGGCGACAGACAGCCGG CTACAACCTGGACCAAGTCCTTGAACAGGGAGGTGTGTCCAGTTTGTT TCAGAATCTCGGGGTGTCCGTAACTCCGATCCAAAGGATTGTCCTGA GCGGTGAAAATGGGCTGAAGATCGACATCCATGTCATCATCCCGTAT GAAGGTCTGAGCGGCGACCAAATGGGCCAGATCGAAAAAATTTTTAA GGTGGTGTACCCTGTGGATGATCATCACTTTAAGGTGATCCTGCACTA TGGCACACTGGTAATCGACGGGGTTACGCCGAACATGATCGACTATT TCGGACGGCCGTATGAAGGCATCGCCGTGTTCGACGGCAAAAAGATC ACTGTAACAGGGACCCTGTGGAACGGCAACAAAATTATCGACGAGCG CCTGATCAACCCCGACGGCTCCCTGCTGTTCCGAGTAACCATCAACG GAGTGACCGGCTGGCGGCTGTGCGAACGCATTCTGGCGGACTACAA AGACCATGACGGTGATTATAAAGATCATGACATCGATTACAAGGATGA CGATGACAAGTAAGGCCGCGACTCTAGAG
ATG-V5-(GGN)₁₀₃-NLuc-3xFLAG	GGAGACCCAAGCTGGCTAGCGTTTAAACTTAAGCTTGGTACCGAGCT CGGATCCACTAGTCCAGTGTGGTGGAATTCGTAGTATGGGTAAGCCT ATCCCTAACCCTCTCCTCGGTCTCGATTCTACGGCGCCGCTGCCAGG GGGCGTGCGGCAGCGCGGTGGCGGAGGAGGTGGGGGTGGTGGAG GAGGAGGCGGTGGTGGAGGTGGTGGGGGAGGAGGCGGAGGAGGG GGTGGTGGTGGCGGCGGTGGAGGAGGAGGCGGTGGTGGTGGAGGA GGCGGAGGAGGAGGGGGTGGAGGAGGAGGTGGCGGTGGTGGTGGA GGAGGCGGAGGAGGAGGTGGCGGTGGTGGAGGTGGCGGTGGAGGG GGCGGTGGTGGTGGAGGTGGGGGAGGTGGTGGAGGAGGTGGTGGC GGTGGAGGTGGAGGCGGTGGAGGAGGAGGTGGCGGAGGCGGTGGT GGAGGAGGAGGTTGGGCCTCGAGGATATCAAGATCTGGCCTCGGCG GCCAAGCTTGGCAATCCGGTACTGTTGGTAAAGCCACCGGGGTCTTC ACACTCGAAGATTTCGTTGGGGACTGGCGACAGACAGCCGGCTACAA CCTGGACCAAGTCCTTGAACAGGGAGGTGTGTCCAGTTTGTTTCAGA ATCTCGGGGTGTCCGTAACTCCGATCCAAAGGATTGTCCTGAGCGGT GAAAATGGGCTGAAGATCGACATCCATGTCATCATCCCGTATGAAGGT CTGAGCGGCGACCAAATGGGCCAGATCGAAAAAATTTTTAAGGTGGT GTACCCTGTGGATGATCATCACTTTAAGGTGATCCTGCACTATGGCAC ACTGGTAATCGACGGGGTTACGCCGAACATGATCGACTATTTCGGAC GGCCGTATGAAGGCATCGCCGTGTTCGACGGCAAAAAGATCACTGTA ACAGGGACCCTGTGGAACGGCAACAAAATTATCGACGAGCGCCTGAT CAACCCCGACGGCTCCCTGCTGTTCCGAGTAACCATCAACGGAGTGA CCGGCTGGCGGCTGTGCGAACGCATTCTGGCGGACTACAAAGACCAT GACGGTGATTATAAAGATCATGACATCGATTACAAGGATGACGATGAC AAGTAAGGCCGCGACTCTAGAG