Figure 2.

Match and mismatch discrimination. (A) A fluorescence labeled oligonucleotide was hybridized against an array containing oligonucleotides with perfect match (row 1), oligonucleotides with two internal mismatches (row 2) or oligonucleotides with an unrelated sequence (row 3). (B) Signal of SYBR Green I staining of the same array after hybridization demonstrating the presence of oligonucleotide probes in all rows. The higher fluorescence signal in the two hybridized rows is probably due to better staining of double-stranded DNA. Both signals are close to saturation. (C) Densitometric evaluation of signals as an average of five spots. The distance between the spots was 200 m.