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. 2000 Jun 15;28(12):e66. doi: 10.1093/nar/28.12.e66

Figure 3.

Figure 3

Detection of immobilized protein with FITC-labeled antibody. (A) Protein antigens were immobilized and subsequently incubated with the labeled antibody. The fluorescence signal of the bound antibody correlates with the concentration of immobilized rhBAD protein on the agarose film-coated slide. In each spot ~0.5–2 nl of the following protein dilutions were spotted: row 1, 0.34 µg/µl; row 2, 0.68 µg/µl; row 3, 1.35 µg/µl; row 4, 2.7 µg/µl rhBAD in spotting solution. (B) Anti-BAD monoclonal antibody was spotted on agarose film. Signals were obtained by incubation with a FITC-labeled second antibody, specific for a different epitope with or without pre-incubation with rhBAD protein. (C and D) Densitometric evaluation of the fluorescence signal. The distance between the spots was 200 m.