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. Author manuscript; available in PMC: 2024 May 15.
Published in final edited form as: J Neurosci Methods. 2023 Apr 26;392:109867. doi: 10.1016/j.jneumeth.2023.109867

Figure 5.

Figure 5.

Expression of TJs, ZO-1, and claudin 5 on human brain cells, layered on polyester transwell insert membrane. ZO-1 (Red) and claudin 5 (Green), and the nucleus (blue) were stained with DAPI (4,6-diamidino-2-phenylindole). TJs were observed on- astrocytes and pericytes in medium alone (a), with the addition of Zn sulfate (b), and hBMECs in medium alone (c) with Zn sulfate (d).

Abbreviations- Zn, zinc; TJ, tight junctions; Z0-1, Zona occludens; hBMCE, Human brain microvascular endothelial cells; DAPI, 4',6-diamidino-2-phenylindole.