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. Author manuscript; available in PMC: 2024 May 15.
Published in final edited form as: J Neurosci Methods. 2023 Apr 26;392:109867. doi: 10.1016/j.jneumeth.2023.109867

Figure 6.

Figure 6.

Cell identification by detecting s100 beta on astrocytes and CD146 on pericytes and hBMECs as respective cell markers. Astrocytes, pericytes, and hBMEc on a transwell membrane, immunostained with a cocktail of s100beta antibody (red), an antibody against CD146 (green), and the nucleus was stained with DAPI (blue). The basolateral surface of the transwell membrane shows astrocyte and pericyte (a), and the apical part of the same transwell membrane shows hBMECs only (b).

Abbreviations- hBMEC, Human brain microvascular endothelial cells; DAPI, 4',6-diamidino-2-phenylindole.