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Expression of ZO-1 (red), claudin 5 (green) in mono-culture of hBMECs on a transwell membrane (a) and determination of claudin 5 on neuron cell from the non-contact 4-cell model with neuronal cell marker in red (b) and when the medium for the BBB model was supplemented with Zn (c).
Abbreviations- Zn, zinc; Z0-1, Zona occludens; hBMEC, Human brain microvascular endothelial cells; DAPI, 4',6-diamidino-2-phenylindole.
