Table 1.

Antibodies used in multiplexed immunohistochemistry

Position	Antibody	Clone/host	Company/item	Concentration μg/mL	OPAL fluor
1	CD3	SP7 / rabbit	Thermo Fisher Scientific / RM-9107	0.06 (1:400)	520
2	CD19	UMAB103 / mouse	Origene / UM500071	0.5 (1:2000)	690
3	CD33	RBT-CD33 / rabbit	Bio SB Inc / BSB3451	1 (1:100)	570
4	CD79A	JCB117 / mouse	Bio SB Inc / BSB5305	0.166 (1:600)	540
5	CD79B	EPR6861 / rabbit	Abcam / ab134147	0.065 (1:1600)	620
6	Cyclin-D	SP4 / rabbit	Sigma / SAB5500090	0.625 (1:400)	650
Secondary	Opal polymer HRP Ms+Rb		Akoya Biosciences / ARH1001EA	Ready to use