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. 2023 Jun 8;3(6):688–704. doi: 10.1038/s43587-023-00431-z

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 1 — a, Flow cytometry plots representative of the sorting strategy followed to obtain CD45+ and CD45–/EpCAM– cells for 10× scRNA-seq. b, UMAP representation of CD45+ Lymphoid (upper panel), CD45+ Myeloid (middle panel) and CD45- (lower panel) cells showing replicate contribution, batch effect correction and good sample mixing calculated with LISI(Korsunsky, Millard et al. 2019). c, Bar plots showing percentage of total cells divided into each cell type per replicate, CD45+ Lymphoid (left panel), CD45+ Myeloid (middle panel) and CD45- (right panel) cells.