Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jun 8;3(6):688–704. doi: 10.1038/s43587-023-00431-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 3 — a,b, Box plots showing changes in proportion of (a) myeloid and (b) lymphoid cells in adult and aged conditions obtained with sccomp(Mangiola 2022) obtained with the scRNA-seq data. Error bars indicate the 95% credible intervals, center line is the median. The black boxplots represent the observed data, while blue ones indicate the posterior predictive check of the model. Boxes colored in orange indicate statistically significant differences in cell proportion between conditions (FDR < 0.025 using Benjamini-Hochberg procedure to control for multiple testing) and red triangles outside the boxes mark outliers. c, Plots of selected GO categories belonging to Biological Processes (BP) analysis for genes upregulated in monocyte_1 cluster (upper panel) and monocyte_2 cluster (lower panel) upon aging. The x axis represents –(log₁₀) of the adjusted P-value for each depicted GO category. d, Violin plots showing expression levels of Il1b in cells belonging to clusters monocyte_1, macrophage_2, and dendritic cells in adult and aged conditions. e, Immunostaining for CD4 in adult (upper panel) and aged (lower panel) back skin. White arrows point to positive cells. Bar = 50 μm. Insets are shown in the right panel next to each condition, bar = 10 μm. * = unspecific hair shaft autofluorescence. N = 5 individual mice. Quantification shows percentage of positive cells. Brightness and contrast were adjusted only for visualization purposes. Quantifications were done in raw images.