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. 2023 Jun 16;6:646. doi: 10.1038/s42003-023-05030-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — In all panels, the black dots with vertical bars represent the mean value and the 95% CI in the Ae. aegypti and Ae. albopictus assays, respectively. The large coloured dots around estimates represent the mean values of each replicate (N). Significant differences between the lines are specified in the figure (n.s.: no significant difference. *p-value < 0.1, **p-value < 0.01, ***p-value < 0.001). The percentages of males were compared using a generalised linear model with a binomial distribution in the Ae. aegypti (a) and Ae. albopictus (e) lines. Comparisons of the mean number of eggs laid by an individual female were analysed using a hurdle model with a negative binomial distribution in the Ae. aegypti (b) and Ae. albopictus (f) lines. Significance of the hurdle model is displayed in two parts: probability of the non-zero values and probability of attaining value 0 (between parentheses). Comparisons of the percentage of egg hatch were analysed using a generalised linear model with a binomial distribution in the Ae. aegypti (c) and Ae. albopictus (g) lines. Comparison of survival from first instar larvae to adult stages were analysed using a linear model with the assumption of residual normality in the Ae. aegypti (d) and Ae. albopictus (h) lines. Figure designed on BioRender.com.