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. 2023 Jun 16;14:3584. doi: 10.1038/s41467-023-39069-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Lay-out of the experimental setup. AM absorbing microdroplets, LB laser beam, UW ultrasound waves, SA spherical array. b Bright field (5×) optical microscopy image of the dichloromethane (DCM) microdroplets along with a histogram of the measured diameters from 500 microdroplets. Scalebar—50 μm. The measurements were repeated 3 times to ensure reproducibility. c OA images of the mouse brain superimposed to the differential (background subtracted) OA (ΔOA) images. Three microdroplets in the azygos pericallosal artery (MD1), middle internal frontal artery penetrating branches (MD2) and pial surface (MD3) are labeled in the differential image. The co-registered Allen mouse brain atlas is also shown. ACA anterior cerebral artery. Scalebar—3 mm, a.u. arbitrary units. Cartesian axes are indicated with arrows. d Maximum intensity projections (MIPs) of the differential OA image along the z and x directions for the rectangular box indicated in c. Three representative time points are shown. The depth from the pia matter (PM) and the calculated velocities are indicated in blue and yellow, respectively. Scalebar—1 mm, a.u. arbitrary units.