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. 2023 Jun 16;14(6):364. doi: 10.1038/s41419-023-05883-0

Fig. 2. CRISPR/Cas9-mediated knockout of TOPK inhibits cell proliferation of EC in vitro and in vivo.

Fig. 2

a, b Expression levels of TOPK protein in TOPK knockout (sgRNA) KYSE150 (a) and KYSE30 (b) cells were measured by Western blot. c, d The effect of TOPK knockout on KYSE150 (c) and KYSE30 (d) cell proliferation was measured by cell proliferation assay. OD values were measured at 0, 24, 48, 72, and 96 h by MTT assay. e, f The effect of TOPK knockout on KYSE150 (e) and KYSE30 (f) cells’ anchorage-independent growth ability was measured by anchorage-independent growth assay. g, h The effect of TOPK knockout on KYSE150 (g) and KYSE30 (h) cells’ anchorage-dependent growth ability was measured by plate clone formation assay. i Expression levels of TOPK protein in TOPK over-expressed KYSE410 cells were measured by Western blot. j The effect of TOPK over-expression on KYSE410 cell proliferation was measured by cell proliferation assay. OD values were measured at 0, 24, 48, 72, and 96 h in MTT assay. k, l The effect of TOPK over-expression on KYSE410 cell’s anchorage-independent growth ability (k) and anchorage-dependent growth ability (l) in KYSE410 were measured by anchorage-independent growth assay and plate clone formation assay, respectively. m. The representative tumor pictures of sgTOPK KYSE30 CDX model. n The tumor growth curves in sgTOPK KYSE30 CDX model. o Tumor weights analysis of sgTOPK KYSE30 CDX model. All experiments were biological replicates and were repeated at least three times. Error bars showed standard error of the mean. *p ˂ 0.05, **p ˂ 0.01, ***p ˂ 0.001.