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. 2023 Jun 16;14:3599. doi: 10.1038/s41467-023-39347-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Demonstration on the accuracy, precision, and linearity of the quantification in the MSx scans using the Pierce Suitability Standard Mix, which is comprised of 7 peptides, each one with 5 isotopologue sequences present in a dilution series ranging from 0.5 pmol/µl to 0.3 fmol/µl. The isotopologue with the highest concentration (Heavy4) was used as a triggering peptide, and the subsequent peptides were used as endogenous counterparts, and were triggered sequentially in the acquisition cycle. RT: retention time. Source data are provided as a Source Data file.