Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jun 16;14:3599. doi: 10.1038/s41467-023-39347-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — a Heatmap showing relative quantification of targeted peptides in a dilution series experiment (Fig. 3a) in SureQuant (pink) and hybrid-DIA (blue). Sites are sorted by abundance. b Boxplots showing the phospho-site ratio of targeted peptides (measured as log2 of the ratio ENDO/IS in one amount versus ratio ENDO/IS in 30 µg) in SureQuant (left) and hybrid-DIA (right) analysis. Each plot shows the ratios between replicates of 30 µg of input material, and 30 µg vs 20 µg, 30 µg vs 10 µg, 30 µg vs 5 µg and 30 µg vs 2.5 µg. Center lines show the medians; box limits indicate the 25th and 75th percentiles as determined by R software; whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles, outliers are represented by dots. N = 80, 84, 82, 77, 79, 76, 76, 70, 72, 63. c Comparative table of characteristics and performance of DIA, hybrid-DIA, and SureQuant based on different aspects of shot-gun and targeted proteomics analysis. Source data are provided as a Source Data file.