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. 2023 Jan 18;91(3):263–280. doi: 10.1007/s00239-022-10083-z

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Barcode design features and error rates. (A) The total indel error rate in homopolymer runs, estimated from barcode data in the datasets indicated in the legend. Barcode designs are shown in Fig. 1 and Table S1. (B) The frequency of homopolymer runs of different lengths in the empirical and simulated datasets of barcodes with different designs (see Methods for details). (C) The total indel error rate in dinucleotide runs, estimated from barcode data. (D) The frequency of dinucleotide runs of different lengths in the empirical and simulated datasets of barcodes with different designs. (E) The empirical distributions of barcode lengths, putatively due to variation in the true length of barcodes (see Methods for details). (F) The distributions of GC content in barcodes in the empirical and simulated datasets (Color figure online)