Fig. 4.

Measurements of media pH when cultured in various conditions. The media pH of WT cultures grown in either LB or DM-1000 broth in tubes made of either plastic (P, orange) or glass (G, cyan), as well as flasks (purple) was measured following 24 h of growth in either still or shaking conditions. In both medias, cultures grown in plastic tubes are acidic in both still and shaking conditions, while cultures grown in glass tubes are more acidic when incubated still than when incubated in shaking conditions. Data points represent three biological replicates and error bars reflect the 95% confidence interval (Color figure online)