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| Reagent | Final concentration | Amount |
|---|---|---|
| HEPES-KOH, pH 7.9 (1 M) | 300 mM | 75 mL |
| Potassium acetate (2.5 M) | 1.4 M | 140 mL |
| Magnesium acetate (1 M) | 30 mM | 7.5 mL |
| DTT (1 M) | 0.5 mM | 125 μL |
| CHAPS (1% (w/v)) | 0.01% (w/v) | 2.5 mL |
| Nuclease-free water | N/A | 24.9 mL |
| Total | N/A | 250 mL |
