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. 2023 Jun 3;4(2):102336. doi: 10.1016/j.xpro.2023.102336
Buffer C
Reagent Final concentration Amount
HEPES-KOH, pH 7.9 (1 M) 30 mM 1.5 mL
Potassium acetate (2.5 M) 120 mM 2.4 mL
Magnesium acetate (1 M) 3.5 mM 175 μL
DTT (1 M) 2 mM 100 μL
CHAPS (1% (w/v)) 0.01% (w/v) 0.5 mL
Glycerol 80% (w/v) 40 g
Nuclease-free water N/A to 50 mL
Total N/A 50 mL