| Reagent | Final concentration | Amount |
|---|---|---|
| HEPES-KOH, pH 7.9 (1 M) | 30 mM | 1.5 mL |
| Potassium acetate (2.5 M) | 120 mM | 2.4 mL |
| Magnesium acetate (1 M) | 3.5 mM | 175 μL |
| DTT (1 M) | 2 mM | 100 μL |
| CHAPS (1% (w/v)) | 0.01% (w/v) | 0.5 mL |
| Glycerol | 80% (w/v) | 40 g |
| Nuclease-free water | N/A | to 50 mL |
| Total | N/A | 50 mL |