Dilution Buffer

Reagent	Final concentration	Amount
HEPES-KOH, pH 7.9 (1 M)	30 mM	30 μL
Potassium acetate (2.5 M)	120 mM	48 μL
Magnesium acetate (1 M)	3.5 mM	3.5 μL
DTT (1 M)	1 mM	1 μL
CHAPS (1% (w/v))	0.01% (w/v)	10 μL
tRNA (2 mg·mL−1)	0.01 mg·mL−1	5 μL
Nuclease-free water	N/A	902.5 μL
Total	N/A	1,000 μL

Prepare immediately before use and store it at 4°C.