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. 2023 Jun 3;4(2):102332. doi: 10.1016/j.xpro.2023.102332

4% Agarose solution (per 50 mL)

Reagent Final concentration Amount
Agarose 4% 2.0 g
dH2O N/A 50 mL

We microwave the solution in a 200 mL flask until the agarose is completely dissolved. The solution can be stored at room temperature with a piece of aluminum foil as a cover. To reuse the agar solution, melt the solidified solution for 35 s in a microwave oven.