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. 2023 Jun 9;4(2):102347. doi: 10.1016/j.xpro.2023.102347

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© 2023 The Authors.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Gating strategy of HSCs

Representative flow cytometric plot for sorting HSCs from UBC-GFP mice. HSCs were defined as CD150⁺CD48^-Flt3^- LSK cells. Multipotent progenitors (MPPs) were sub-fractionated into MPP1 (CD150^-CD48^-Flt3^- LSK), MPP2 (CD150⁺CD48⁺Flt3^- LSK), MPP3 (CD150^-CD48⁺Flt3^- LSK), and MPP4 (Flt3⁺ LSK).