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. 2023 Jun 9;4(2):102347. doi: 10.1016/j.xpro.2023.102347

Figure 3.

Figure 3

Primer design for evaluating genome editing efficiency

(A) Design of sequence primer sets for sgRNA targeting of the UBC-GFP locus.

(B) Representative Sanger sequencing results of non-edited and GFP-edited cells.

(C) Results of a TIDE assay.

(D) Results of ICE analysis.

(E) Genome editing efficiency based on ICE positively correlated with GFP expression levels. The donor-derived peripheral blood cells from individual recipient mice in two independent transplant experiments were used for ICE analysis (mean ± SD, n = 10).

(F) The ratio of genome editing efficiency at the transplant to genome editing efficiency at four months after transplant. The same data set is used as in Figure 2E (mean ± SD, n = 10).