BSA (4%) medium

Reagent	Stock concentration	Final concentration	Amount
DMEM/F12	–	N/A	9980 μL
BSA	–	4%	400 mg
Palmitic acid	16 mg/mL	100 μg/mL	62.5 μL
Oleic acid	30 mg/mL	100 μg/mL	33 μL
Cholesterol	4 mg/mL	20 μg/mL	50 μL
2-ME	55 mM	55 μM	10 μL
IST mixture	N/A	N/A	10 μL
Total	N/A	N/A	10 mL