| Reagent | Stock concentration | Final concentration | Amount |
|---|---|---|---|
| 4% BSA medium | – | – | 197.5 μL |
| Stem cell factor (SCF) | 20 ng/μL | 150 ng/mL | 1.5 μL |
| Thrombopoietin (TPO) | 20 ng/μL | 100 ng/mL | 1 μL |
| Total | N/A | N/A | 200 μL |
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