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. 2023 Jun 9;4(2):102347. doi: 10.1016/j.xpro.2023.102347

Quiescence-maintaining culture

Reagent Stock concentration Final concentration Amount
4% BSA medium 9900 μL
Cytokine master stock SCF 150 ng/mL
TPO 100 ng/mL
SCF 1.5 ng/mL
TPO 1.0 ng/mL
100 μL
Total N/A N/A 10 mL