sgRNA synthesis (CUGA7 system)
| Reagent | Amount |
|---|---|
| 5x Transcriptional buffer | 4 μL |
| 0.1 M DTT | 2 μL |
| NTP Mix | 6 μL |
| DNA template | 7 μL |
| CUGA7 Enzyme Solution | 1 μL |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.