PCR reaction master mix
| Reagent | Amount |
|---|---|
| DNA template | 1 μL |
| Q5 High-Fidelity DNA Polymerase | 12.5 μL |
| UBC-GFP Forward Primer (5μM) | 2.5 μL |
| UBC-GFP Reverse Primer (5μM) | 2.5 μL |
| ddH2O | 6.5 μL |
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