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. 2002 Apr 9;3:9. doi: 10.1186/1471-2164-3-9

Figure 1.

Figure 1

Yeast two-hybrid interaction of LeFPP with MAF1. (A)Yeast colonies co-expressing bait and prey plasmids were streaked on dropout plates without leucine and tryptophane (LT) or dropout plates without leucine, tryptophane, and histidine (LTH). (a) pBD-LeMAF1/pAD-LeFPP; (b) pBD-AtMAF1/pAD-LeFPP; (c) pBD-AtRanGAP1/pAD-LeFPP; (d) pBD-GAL4/pAD-LeFPP. (B) Quantification of β-Galactosidase activity in yeast strains harboring combinations of bait and prey plasmids. (1) p53/pSV40 (positive control); (2) pLaminC/pSV40 (negative control); (3) pBD-LeMAF1/pAD-LeFPP; (4) pBD-GAL4/pAD-LeFPP. Units, relative β-Galactosidase units (see Methods). (C) Sequence alignment between LeMAF1, AtMAF1, and the N-terminal domain of AtRanGAP1. Identical amino acids are shaded in black.