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. Author manuscript; available in PMC: 2023 Jun 17.
Published in final edited form as: Anal Chem. 2022 Dec 13;94(51):17895–17903. doi: 10.1021/acs.analchem.2c03844

Figure 1. Design of biotin thiol tag for citrullination analysis.

Figure 1.

a, Derivatization of citrullinated peptides using biotin thiol tag and 2,3-butanedione. b, Fragmentation sites of biotin thiol tag-derivatized citrullinated peptides upon HCD, ETD, or EThcD fragmentations. c, Tandem MS spectrum of the biotin thiol tag-derivatized citrullinated peptide standard SAVRACitSSVPGVR upon HCD fragmentation.