a, Derivatization of citrullinated peptides using biotin thiol tag and 2,3-butanedione. b, Fragmentation sites of biotin thiol tag-derivatized citrullinated peptides upon HCD, ETD, or EThcD fragmentations. c, Tandem MS spectrum of the biotin thiol tag-derivatized citrullinated peptide standard SAVRACitSSVPGVR upon HCD fragmentation.